Plasmid Preparation

The standard method is to use GFX plasmid purification column from Amersham/Pharmacia. But for a more traditional method see below:

Notes- 0.5µl of a plasmid prep is usually more than sufficient for an agarose gel loading. This method may be scaled up or down as required. 2µl of these plasmid preps may be used directly for ABI sequencing.