l phage DNA preparation


BULK GROWTH OF l PHAGE AND PURIFICATION OF ITS DNA

Day 1

Phage plate stock
 


Alternatively collect 6-10 positive plaques and pool in 0.5ml of SM and 2oµl chloroform and use this as a stock.

Bulk phage growth
 


Phage purification
 

Reagents

LB media; 10g tryptone, 5g yeast extract, 5g NaCl

LB agar; LB media with 10g/l agar

LB top agarose; LB media with 0.6g/100ml agarose

SM 10mM TRIS/HCl, pH 7.4, 10mM MgSO4, 0.01% gelatin